Brucellosis is a worldwide infectious disease that arises from Brucella type bacteria. The infection is transmitted to humans through the consumption of unpasteurized milk, direct contact with infected animals. Congenital brucellosis occurs using trans-placental transmission due to maternal infection during pregnancy or ingestion of the contaminated maternal secretions during birth and breastfeeding. The diagnosis of brucellosis is made by culture or serology. In this article, the case of a premature infant with congenital brucellosis presumed to be acquired trans-placental transmission is reported because of the rarity of such cases. We should note that preventive procedures should be taken to protect pregnant women living in high-risk areas where brucellosis is endemic.Keywords: Brucellosis, intrauterine infection; preterm newborn.