Investigation of Antiproliferative, Apoptotic, and Migration Activity of Combination of Paclitaxel and Aloe Vera in Non-Small Cell Lung Cancer and Breast Cancer
Tuğba Kul Köprülü1, Jülide Balkan21Experimental Medicine Application and Research Center, Validebağ Research Park, University of Health Sciences Türkiye, İstanbul, Türkiye; Department of Medical Services and Techniques, University of Health Sciences, Hamidiye Vocational Faculty of Health Services, Medical Laboratory Techniques, İstanbul, Türkiye2Department of Molecular Medicine, University of Health Sciences Türkiye, Hamidiye Institute of Health Sciences, Istanbul, Türkiye
INTRODUCTION: The aim of this study is to determine whether it is possible to increase the cytotoxic effects of known anti-cancer agents with natural compounds. For this purpose, the antiproliferative effect of Aloe vera (AVE) against human breast cancer (MCF-7) and lung cancer (A549) and the in vitro potential anticancer activity of its combination with Paclitaxel (PAX) were investigated.
METHODS: The antiproliferative activity of the AVE and AVE-PAX combination was assessed against MCF-7 and A549 cancer cell lines and non-cancer cell (BEAS-2B) using xCELLigence real-time cell analysis. Cell apoptosis was determined by DNA laddering assay and Annexin V/FITC flow cytometer, and cell migration was evaluated by wound healing assay.
RESULTS: Treatment with the AVE and AVE-PAX significantly increased the antiproliferative activity in A549, MCF-7, and BEAS-2B cells compared to the control group (p<0.05). AVE-PAX combination administration resulted in earlier antiproliferative activity than AVE in all three cell lines (p<0.05). It was determined that when 24 µg/mL AVE and 5 µM PAX were applied together, it triggered the apoptotic process in A549 and MCF-7 cells and showed an anti-migration effect.
DISCUSSION AND CONCLUSION: In our study, the combination of AVE-PAX reduced the growth and cell migration of MCF-7 and A549 cells with low PAX susceptibility in vitro and induced early apoptosis of A549 and MCF-7 cells. These results show that the use of AVE together with PAX is effective in NSCLC and breast cancer and has a synergistic effect.
Manuscript Language: English