Haydarpasa Numune Med J. 2020; 60(3): 250-253 | DOI: 10.14744/hnhj.2020.81488

The Role of TNF-alpha and IL-10 Cytokines in Degenerative Lumbar Spinal Stenosis

Mustafa Efendioðlu¹, Emine Emel Koçman^2
1Department of Neurosurgery, University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Haydarpaþa Numune Health Application and Research Center, Istanbul, Turkey
²Department of Basic Immunology, University of Health Sciences Turkey, Hamidiye Faculty of Medicine, Haydarpaþa Numune Health Application and Research Center, Istanbul, Turkey

INTRODUCTION: In this study, we investigated Tumor Necrosis Factor-α (TNF-α)/Interleukin -10 (IL-10) (inflammatory/anti-inflammatory) cytokine balance in patients diagnosed with degenerative Lumbar Spinal Stenosis (LSS).
METHODS: Blood samples obtained from patients and control subjects were centrifuged at the Haydarpaþa Numune Training and Research Hospital Immunology Laboratory and stored at -20°C. Human TNF-alpha and IL-10 cytokines were studied manually with an Enzyme-linked immunosorbent assay (ELISA) method using Boster brand 96-well ELISA kits.
RESULTS: TNF -alpha values of the patient group were found to be statistically significantly higher than the control group (p=0.004; p<0.05) and IL-10 values of the patient group were statistically significantly higher than the control group (p=0.017; p<0.05).
DISCUSSION AND CONCLUSION: Our findings suggest that TNF-alpha and IL-10 cytokine levels increased together in degenerative LSS cases. The inflammatory process of the disease is trying to be balanced with an anti-inflammatory response

Keywords: Cytokine, IL 10; lumbar spinal stenosis; TNF alpha.

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Dejeneratif lomber spinal stenozda TNF-alfa ve IL-10 sitokinlerinin rolü

Mustafa Efendioðlu¹, Emine Emel Koçman^2
1Saðlýk Bilimleri Üniversitesi, Haydarpaþa Numune Eðitim ve Araþtýrma Hastanesi, Beyin Cerrahisi Bölümü, Ýstanbul
²Saðlýk Bilimleri Üniversitesi, Haydarpaþa Numune Eðitim ve Araþtýrma Hastanesi, Temel Ýmmünoloji Bölümü, Ýstanbul

GÝRÝÞ ve AMAÇ: Bu çalýþmada dejeneratif Lombar Spinal Stenoz (LSS) tanýsý konmuþ hastalarda Tümör Nekroz Faktörü-α (TNF-α)/ Intelökin-10 (IL-10) (enflamatuvar / antienflamatuvar) sitokin dengesini araþtýrdýk.

YÖNTEM ve GEREÇLER: Hasta ve kontrol kanlarý, Haydarpaþa Numune Eðitim ve Araþtýrma Hastanesi Ýmmünoloji Laboratuarýnda santrifüjlenerek -20°C’de saklandý. Enzyme-linked immunosorbant assay (ELISA) yöntemi ile Human TNF-alfa ve IL-10 sitokinleri, Boster marka 96 kuyucuklu ELISA Kitler kullanýlarak manuel yöntemle çalýþýldý.
BULGULAR: Hasta grubunun TNF alfa deðerleri, kontrol grubundan istatistiksel olarak anlamlý düzeyde yüksek bulundu (p: 0.004; p<0.05) ve hasta grubunun IL-10 deðerleri, kontrol grubundan istatistiksel olarak anlamlý düzeyde yüksek bulundu (p: 0.017; p<0.05).
TARTIÞMA ve SONUÇ: Kanatimize göre; dejeneratif LSS olgularýnda TNF-alfa ve IL-10 sitokin düzeylerinin birlikte yükselmesi sayesinde; hastalýðýn enflamatuvar süreci, anti-enflamatuvar cevapla dengelenmeye çalýþmaktadýr.

Anahtar Kelimeler: Lumber spinal stenoz, sitokin, TNF alfa, IL 10

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